yrbD [2020-10-27 12:20:49]

sodium/proton-dependent alanine transporter

locus: BSU_27810

pI: 9.38

mw: 51.43 kDa

protein length: 484 aa

gene length: 1455 bp

function: uptake of alanine

product: sodium/proton-dependent alanine transporter

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.1|Amino acid transporters] → [category|SW 1.2.4.1.3|Alanine or glycine cation symporter family]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.8|Biosynthesis/ acquisition of serine/ glycine/ alanine]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.7|Utilization of alanine/ serine]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]

Gene

Coordinates: 2,841,611 2,843,065

The protein

Protein family

• [SW|Alanine or glycine:cation symporter (AGCS) (TC 2.A.25) family] (according to UniProt)

Paralogous protein(s)

• [protein|A69E7536DFE6353C4CD1B09D1258C88BF0FB8B4D|YflA], [protein|B84BE9F5BEFE3A295ED5580D66040CD28609A6A1|AlsT], [protein|E371BC4F613C40CB0E8C8CAA97387F3E6735D813|GlnT]

Structure

• [PDB|6CSE] (sodium/alanine symporter from Methanococcus maripaludis S2, 37% identity) [pubmed|30659158]

Expression and Regulation

Operons

• genes: [gene|A10EA56A7EDA2DF712B5BB87D94A818E39940B44|yrbD]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA]: activation, in [regulon|857EF1AC54DEA0690C01B7F8CEFFD16312C58F20|TnrA regulon]

Biological materials

Mutant

• GP3556 Δ[gene|A10EA56A7EDA2DF712B5BB87D94A818E39940B44|yrbD]::phleo, available in [SW|Jörg Stülke]'s lab
• MGNA-A821 (yrbD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/821 NBRP B. subtilis, Japan]
• BKE27810 ([gene|A10EA56A7EDA2DF712B5BB87D94A818E39940B44|yrbD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27810 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CATCAAGCTTCCCCCTCACT, downstream forward: _UP4_TGAACATACTAAAACCGGCC
• BKK27810 ([gene|A10EA56A7EDA2DF712B5BB87D94A818E39940B44|yrbD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27810 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAGCTTCCCCCTCACT, downstream forward: _UP4_TGAACATACTAAAACCGGCC

References

• 25755103,30659158